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Comparing brain‐derived neurotrophic factor and ciliary neurotrophic factor secretion of induced neurotrophic factor secreting cells from human adipose and bone marrow‐derived stem cells
Author(s) -
Razavi Shahnaz,
Razavi Mohamad Reza,
Zarkesh Esfahani Hamid,
Kazemi Mohammad,
Mostafavi Fatemeh Sadat
Publication year - 2013
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12072
Subject(s) - ciliary neurotrophic factor , neurotrophic factors , brain derived neurotrophic factor , stem cell , mesenchymal stem cell , adipose tissue , bone marrow , neurotrophin , biology , endocrinology , medicine , immunology , microbiology and biotechnology , cancer research , receptor
Adipose derived stem cells ( ADSC s) and bone marrow stem cells ( BMSC s) may be equally beneficial in treating neurodegenerative diseases. However, ADSC s have practical advantages. In this study, we aimed to induce neurotrophic factors secreting cells in human ADSC s. Then, we compared the level of brain‐derived neurotrophic factor ( BDNF ) and ciliary neurotrophic factor ( CNTF ) secretion in neurotrophic factors secreting cells from human adipose and bone marrow‐derived stem cells. Isolated human ADSC s and BMSC s were induced to neurotrophic factor ( NTF )‐secreting cells. The levels of expression and secretion of BDNF and CTNF of induced cells were assessed using immunocytochemical, Real‐Time polymerase chain reaction, and enzyme linked immunosorbent assay ( ELISA) . The level of BDNF significantly increased in both the induced mesenchymal stem cells ( MSC s) relative to ADSC s and the BMSC s ( P < 0.01). Moreover, ELISA analysis showed that the release of BDNF in the induced BMSC s was almost twofold more than the induced ADSC s. Overall, NTF ‐secreting factor cells derived BMSC s and ADSC s could secret a range of different growth factors. Therefore, the variation in neurotrophic factors of different induced MSC populations suggest the possible beneficial effect of each specific kind of neurotrophic factor secreting cells for the treatment of a particular neurodegenerative disease.