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Live‐cell analysis of plant reproduction: Live‐cell imaging, optical manipulation, and advanced microscopy technologies
Author(s) -
Kurihara Daisuke,
Hamamura Yuki,
Higashiyama Tetsuya
Publication year - 2013
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12040
Subject(s) - sexual reproduction , plant reproduction , reproduction , live cell imaging , biology , human reproduction , microbiology and biotechnology , cell , ecology , anatomy , genetics , pollination , pollen
Sexual reproduction ensures propagation of species and enhances genetic diversity within populations. In flowering plants, sexual reproduction requires complicated and multi‐step cell‐to‐cell communications among male and female cells. However, the confined nature of plant reproduction processes, which occur in the female reproductive organs and several cell layers of the pistil, limits our ability to observe these events in vivo . In this review, we discuss recent live‐cell imaging in in vitro systems and the optical manipulation techniques that are used to capture the dynamic mechanisms representing molecular and cellular communications in sexual plant reproduction.