Premium
Analysis of localization and reorganization of germ plasm in X enopus transgenic line with fluorescence‐labeled mitochondria
Author(s) -
Taguchi Ayaka,
Tak Miki,
Motoishi Minako,
Or Hidefumi,
Moch Makoto,
Watanabe Kenji
Publication year - 2012
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/dgd.12005
Subject(s) - germ plasm , xenopus , biology , microbiology and biotechnology , cytoplasm , germ line development , green fluorescent protein , mitochondrion , germ cell , transgene , somatic cell , embryo , biochemistry , gene
Germ plasm is found in germ‐line cells of X enopus and thought to include the determinant of primordial germ cells ( PGC s). As mitochondria is abundant in germ plasm, vital staining of mitochondria was used to analyze the movement and function of germ plasm; however, its application was limited in early cleavage embryos. We made transgenic X enopus , harboring enhanced green fluorescent protein ( EGFP ) fused to the mitochondria transport signal ( D ria‐line). Germ plasm with EGFP ‐labeled mitochondria was clearly distinguishable from the other cytoplasm, and retained mostly during one generation of germ‐line cells in D ria‐line females. Using the D ria‐line, we show that germ plasm is reorganized from near the cell membrane to the perinuclear space at S t. 9, dependent on the microtubule system.