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An automated quantitative DNA image cytometry system detects abnormal cells in cervical cytology with high sensitivity
Author(s) -
Wong O. G.,
Ho M. W.,
Tsun O. K.,
Ng A. K.,
Tsui E. Y.,
Chow J. N.,
Ip P. P.,
Cheung A. N.
Publication year - 2018
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1111/cyt.12540
Subject(s) - squamous intraepithelial lesion , medicine , genotyping , cytology , cervical intraepithelial neoplasia , biopsy , liquid based cytology , pathology , squamous carcinoma , cervical cancer , carcinoma , gynecology , cancer , genotype , biology , gene , biochemistry
Objective To evaluate the performance of an automated DNA ‐image‐cytometry system as a tool to detect cervical carcinoma. Methods Of 384 liquid‐based cervical cytology samples with available biopsy follow‐up were analyzed by both the Imager System and a high‐risk HPV test (Cobas). Results The sensitivity and specificity of Imager System for detecting biopsy proven high‐grade squamous intraepithelial lesion ( HSIL , cervical intraepithelial neoplasia [ CIN] 2‐3) and carcinoma were 89.58% and 56.25%, respectively, compared to 97.22% and 23.33% of HPV test but additional HPV 16/18 genotyping increased the specificity to 69.58%. The sensitivity and specificity of the Imager System for predicting HSIL + ( CIN 2‐3+) lesions among atypical squamous cells of undetermined significance samples were 80.00% and 70.53%, respectively, compared to 100% and 11.58% of HPV test whilst the HPV 16/18 genotyping increased the specificity to 77.89%. Among atypical squamous cells‐cannot exclude HSIL , the sensitivity and specificity of Imager System for predicting HSIL + ( CIN 2‐3+) lesions upon follow up were 82.86% and 33.33%%, respectively, compared to 97.14% and 4.76% of HPV test and the HPV 16/18 genotyping increased the specificity to 19.05%. Among low‐grade squamous intraepithelial lesion cases, the sensitivity and specificity of the Imager System for predicting HSIL + ( CIN 2‐3+) lesions were 66.67% and 35.71%%, respectively, compared to 66.67% and 29.76% of HPV test while HPV 16/18 genotyping increased the specificity to 79.76%. The overall results of imager and high‐risk HPV test agreed in 69.43% (268) of all samples. Conclusions The automated imager system and HPV 16/18 genotyping can enhance the specificity of detecting HSIL + ( CIN 2‐3+) lesions.