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Availability of immunocytochemistry using cocktail antibody targeting p63/cytokeratin14 for the differential diagnosis of fibroadenoma and ductal carcinoma in situ in fine needle aspiration cytology of the breast
Author(s) -
Maeda I.,
Oana Y.,
Tsugawa K.,
Takagi M.
Publication year - 2017
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1111/cyt.12434
Subject(s) - medicine , fibroadenoma , ductal carcinoma , pathology , differential diagnosis , immunocytochemistry , papanicolaou stain , staining , biopsy , breast cancer , radiology , cancer , cervical cancer
Objective The differential diagnosis of fibroadenoma ( FA ) and ductal carcinoma in situ ( DCIS ) has been problematic in fine needle aspiration biopsy ( FNAC ) because it has been difficult to differentiate between the “large epithelial clusters” associated with FA and those associated with DCIS . The purpose of this study was to prospectively validate the usefulness of immunocytochemical staining using cocktail antibody targeting p63/ CK 14 in the differential diagnosis of FA and DCIS .Materials and methods Twenty patients diagnosed as having an uncertain malignant potential (indeterminate) for breast cancer on the basis of a FNAC finding were selected randomly: ten patients with FA and ten with DCIS . The cover glass on a specimen stained with the Papanicolaou stain on a glass slide was peeled off, and the specimen was restained by immunocytochemical staining of cocktail antibody targeting p63 and CK 14.Results Six of the twenty patients were CK 14‐immunopositive: FA , 6; DCIS , 0. The remaining patients were CK 14‐immunonegative: FA , 4; DCIS , 10. The number of CK 14‐immunopositive DCIS patients was significantly different from that of FA patients ( P =.0054). Eight out of the twenty patients were p63‐immunopositive: FA , 8; DCIS , 0. The remaining patients were p63‐immunonegative: FA , 2; DCIS , 10. The number of p63‐immunopositive DCIS patients was significantly different from that of FA patients ( P =.0004).Conclusions Immunocytochemical staining using cocktail antibody targeting p63/ CK 14 was useful for the differential diagnosis of FA and DCIS in FNAC of the breast.