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Immunoglobulin heavy and light chains and T‐cell receptor beta and gamma chains PCR assessment on cytological samples. A study comparing FTA cards and cryopreserved lymph node fine‐needle cytology
Author(s) -
Peluso A. L.,
Cozzolino I.,
Bottiglieri A.,
Lucchese L.,
Di Crescenzo R. M.,
Langella M.,
Selleri C.,
Zeppa P.
Publication year - 2017
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1111/cyt.12402
Subject(s) - lymph node , cryopreservation , immunoglobulin light chain , polymerase chain reaction , antibody , pathology , medicine , microbiology and biotechnology , biology , immunology , gene , embryo , biochemistry
Objectives To evaluate and compare the DNA yield and quality extracted from lymph node fine needle cytology ( FNC ) samples stored on FTA cards to those cryopreserved, and to assess the immunoglobulin heavy and light chains ( IGHK ) and T‐Cell receptor beta and gamma chains ( TCRBG ) PCR tests. Methods DNA extractions were performed on FNC of 80 non‐Hodgkin lymphomas ( NHL ), four myelomas and 56 benign reactive hyperplasias ( BRH ) cryopreserved and stored on FTA cards. The JAK 2 gene was amplified to assess the DNA integrity and the IGHK / TCRBG clonality status was tested. Results IGHK monoclonality was found in 99% of B‐cell NHL and 100% of myeloma. TCRBG monoclonality was found in 100% of T‐cell NHL . TCRBG polyclonality was detected in 97% of B‐cell NHL , 100% of myeloma and 96% of BRH . IGHK / TCRBG PCR data were confirmed by histological and/or follow‐up controls. No differences were found in the DNA quality between cryopreservation and FTA cards storage methods. Conclusions IGHK / TCRBG PCR of the lymphoproliferative process on FTA cards is comparable to those cryopreserved. FTA cards can be used to store lymph node FNC for further molecular investigations.