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Immunocytochemical analysis of p63 and 34βE12 in fine needle aspiration cytology specimens for breast lesions: a potentially useful discriminatory marker between intraductal papilloma and ductal carcinoma in situ
Author(s) -
Hoshikawa S.,
Sano T.,
Hirato J.,
Oyama T.,
Fukuda T.
Publication year - 2016
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1111/cyt.12244
Subject(s) - intraductal papilloma , medicine , pathology , ductal carcinoma , fine needle aspiration , cytology , carcinoma , immunostaining , papilloma , nipple discharge , carcinoma in situ , immunohistochemistry , breast cancer , biopsy , cancer , mammography
Objective We applied immunocytochemistry to fine needle aspiration ( FNA ) breast lesion slides in an attempt to enhance their objectivity and specificity. Methods We analysed 56 FNA specimens from patients with histologically confirmed breast lesions, using 34βE12 and p63 antibodies. Immunostained slides were examined in terms of both solitary positive cells (within clusters and non‐clusters) and positive clusters within a slide. Results Positive scores (≥2) for p63 + cells and percentages of p63 + clusters differed significantly ( P < 0.001) between malignant (3 of 34; 9%) and benign (11 of 22; 50%) cases and varied between benign and malignant groups: intraductal papilloma ( IDP ) (2 of 8), other benign lesions (9 of 14), ductal carcinoma in situ ( DCIS ) (1 of 11) and invasive carcinoma ( IC ) (2 of 23). Similarly, 34βE12 scores were higher in benign cases ( IDP , 8 of 8; other benign, 9 of 14) than in malignant cases ( DCIS , 1 of 11; IC , 3 of 23). As well as a significant difference between benign and malignant cases (17 of 22, 77% versus 4 of 34, 12%; P < 0.001), the percentage of 34βE12 + clusters was significantly higher in IDP compared with DCIS ( P = 0.001). Conclusions The immunostaining of FNA breast specimens for p63 and 34βE12 may help in difficult diagnoses.