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WT 1 immunoenzyme staining using SurePath ™ processed urine cytology helps to detect kidney disease
Author(s) -
Ohsaki H.,
Sofue T.,
Kawakami K.,
Nishijima Y.,
Hara T.,
Matsunaga T.,
Kushida Y.,
Haba R.,
Shigematsu Y.,
Irino S.,
Norimatsu Y.
Publication year - 2016
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1111/cyt.12227
Subject(s) - urine cytology , medicine , urine , urinary system , kidney , cytology , glomerulonephritis , pathology , nephropathy , kidney disease , minimal change disease , staining , lupus nephritis , gastroenterology , focal segmental glomerulosclerosis , disease , endocrinology , cystoscopy , diabetes mellitus
Objectives Damage and detachment of podocytes and loss into the urine have been implicated in the progression of kidney diseases. The purpose of this study was to investigate the potential role of urine cytology based on SurePath ™ combined with immunoenzyme staining using Wilms’ tumour 1 ( WT 1) antibody as a podocyte marker in the discrimination of normality and non‐renal urinary tract disease from kidney disease. Methods Sixty‐six patients with kidney disease, 45 patients with lower urinary tract disease and 30 healthy volunteers were examined. Urine cytology slides were prepared using the SurePath method and immunoenzyme stained with WT 1 antibody, and the number of WT 1‐positive cells was counted. Results In kidney disease, WT 1‐positive cells were found in 33 (50%) of 66 samples. No WT 1‐positive cells were found in 45 patients with lower urinary tract disease or in 30 healthy volunteers. The positive rates for WT 1 varied with disease type, but not significantly: immunoglobulin A (IgA) nephropathy, (14/23); membranous glomerulonephritis, (4/10); Henoch–Schönlein purpura nephritis, (3/5); diabetic glomerulopathy, (5/5); minor glomerular abnormality/minimal change nephrotic syndrome (0/4). Conclusions The results suggest that WT 1 immunoenzyme staining of urine cytology can be used to detect some types of kidney disease.