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Simultaneous immunostaining with anti‐S100P and anti‐ SV 40 antibodies revealed the origin of BK virus‐infected decoy cells in voided urine samples
Author(s) -
Ariyasu S.,
Yanai H.,
Sato M.,
Shinno Y.,
Taniguchi K.,
Yamadori I.,
Miki Y.,
Sato Y.,
Yoshino T.,
Takahashi K.
Publication year - 2015
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1111/cyt.12213
Subject(s) - immunostaining , bk virus , urine cytology , medicine , urine , transplantation , staining , antibody , pathology , polyomavirus infections , cytology , kidney transplantation , virology , immunohistochemistry , immunology , cystoscopy , alternative medicine
Background Methods for determining the origin of BK virus ( BKV )‐infected cells (decoy cells) in clinical urine samples have not been established although they could enhance the diagnosis of BKV infection in immunocompromised patients. Methods We performed simultaneous immunostaining with anti‐S100P (a urothelial marker) and anti‐ SV 40 antibodies in 66 clinical urine samples exhibiting SV 40 positivity and a decoy‐cell appearance on Papanicolaou staining. The clinical voided urine samples included seven cases of renal transplantation, 47 cases of cancer therapy and 12 cases of non‐neoplastic disease. SurePath ™ liquid‐based cytology was used for the urine samples. Results BKV ‐infected cells were categorized as SV 40(+)/S100P(+) and SV 40 (+)/S100p(−). SV 40(+)/S100P(−) cells were found in 55 cases (83.4%); nine cases (13.6%) carried both SV 40(+)/S100P(−) and SV 40(+)/S100P(+) cells. The former were identified as BKV infection in renal tubules and the latter in both the renal tubules and urothelial epithelia. The remaining two cases (3.0%) had only SV 40(+)/S100P(+) cells of urothelial origin. Conclusion Simultaneous immunostaining with anti‐S100P and anti‐ SV 40 is a useful method for determining the origin of BKV ‐infected cells in clinical urine samples from immunocompromised patients such as renal transplantation recipients.