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Increasing the sensitivity of endocervical curettings by performing ThinPrep ® Pap on transport container fluid: is diagnostic material going down the drain?
Author(s) -
Lastra R. R.,
Meykler S. E.,
Baloch Z. W.,
Barroeta J. E.
Publication year - 2015
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1111/cyt.12177
Subject(s) - medicine , curettage , endocervical curettage , cytology , nuclear medicine , squamous intraepithelial lesion , liquid based cytology , histology , cervical intraepithelial neoplasia , surgery , pathology , cervical cancer , cancer
Objective The sensitivity of endocervical curettage ( ECC ) can be suboptimal because of limited epithelial tissue. The false‐negative rate for ECC in patients with cervical intraepithelial neoplasia involving the endocervical canal has been reported to be 45%. ECC samples are transported to pathology in formalin‐ or saline‐filled containers; this fluid is discarded after the specimen has been submitted. We evaluated the utility of performing liquid‐based cytological preparations from ECC transport container fluid as a way to increase the sensitivity of ECC specimens. Methods Consecutive ECC specimens received at one of the two participating institutions were selected prospectively. A surgical pathology mesh bag was placed over a ThinPrep ® CytoLyt ® solution container, and the specimen was filtered through the bag, collecting the transport fluid in the container. The CytoLyt ® was processed to obtain a container fluid ThinPrep ® ( CF ‐ TP ) liquid‐based Papanicolaou (Pap) slide. The CF ‐ TP slides were reviewed and the findings were compared with those from the ECC and follow‐up specimens. Results The cohort included 53 patients. Discrepancies between CF ‐ TP and ECC were seen in 14 of the 53 patients (26%); a more significant lesion was identified in CF ‐ TP relative to ECC in 13 of these cases. CF ‐ TP diagnosis was confirmed in eight of 11 cases with histological follow‐up. A positive CF ‐ TP result was confirmed by histology in six of nine cases with negative ECC . Conclusions Combining the pathological evaluation of ECC specimens with liquid‐based cytology performed on the transport container fluid can increase the diagnostic sensitivity of the ECC procedure for the detection of cervical lesions.