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Lymph node fine needle aspiration cytology in systemic mastocytosis: cytological features with ancillary tests and literature review
Author(s) -
Cozzolino I.,
Picardi M.,
Lucci R.,
Petraroli A.,
Spadaro G.,
Marone G.,
Vetrani A.,
Zeppa P.
Publication year - 2015
Publication title -
cytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 48
eISSN - 1365-2303
pISSN - 0956-5507
DOI - 10.1111/cyt.12136
Subject(s) - medicine , fine needle aspiration , pathology , lymph node , lymph , immunocytochemistry , cytology , cytopathology , histiocyte , flow cytometry , mast cell , biopsy , immunology
Objective Systemic mastocytosis ( SM ) may rarely involve the lymph nodes; the corresponding cytological features are variable and have rarely been described. The aim of this study was to define more clearly the corresponding cytological diagnostic criteria. Methods Ultrasound‐guided fine needle aspiration ( FNA ) of the deep‐seated lymph nodes of two men, aged 72 and 75 years respectively, was performed with rapid on‐site evaluation ( ROSE ). Additional passes were used to prepare alcohol‐fixed smears for immunocytochemistry ( ICC ) and cell suspensions for flow cytometry ( FC ). Results Smears showed medium‐sized, roundish or elongated, mast cells with round to oval nuclei, sometimes irregular in shape. Cytoplasm was abundant with coarse granules, magenta‐stained on Diff‐Quik ® smears. Mature lymphocytes, plasma cells, histiocytes and a variable number of eosinophils were present in the background. ICC showed mast cell positivity for CD 117 in both cases; FC demonstrated CD 2/ CD 25 co‐expression in one case. On the basis of the cytological and phenotypical features, the diagnosis of lymph node involvement in SM was made in both cases. Cytological ICC and FC data were evaluated using information reported in the literature. Conclusions Lymph node involvement in SM may be detected by FNA and ancillary techniques successfully applied to demonstrate the mast cell phenotype. FNA may also be used in a multidisciplinary algorithm for timely and effective SM staging.