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p16 immunostaining in keratinocytic neoplasia in organ transplant recipients: Bowen's disease shows a characteristic pattern
Author(s) -
Genders Roel E.,
Beck Samuel,
Bouwes Bavinck Jan Nico,
van den Munckhof Henk A.M.,
Kouwenhoven Stijn T.P.,
de Koning Maurits N.C.,
de Gruijl Frank R.,
Jenkins David,
Willemze Rein,
Quint Koen D.
Publication year - 2017
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/cup.12840
Subject(s) - immunostaining , pathology , bowen's disease , staining , immunohistochemistry , medicine , basal cell
Background For selecting therapy, it is important to distinguish different types of keratinocytic neoplasia. It is sometimes difficult to make histopathologic diagnosis, especially in organ transplant recipients ( OTR ) who develop numerous lesions. Methods To investigate p16 immunostaining in different types of keratinocytic neoplasia in OTR , we studied 59 actinic keratoses ( AK ), 51 Bowen' s disease ( BD ), 63 squamous cell carcinomas ( SCC ), 16 benign keratotic lesions ( BKL ) from 31 OTR patients and 25 controls (eczema and psoriasis). Tissue sections were stained for H&E and p16. We scored intensity, proportion and distribution of p16 positive lesional cells. Results In 19% of AK , 92% of BD , 35% of SCC and 12% of BKL more than 15% of lesional cells were p16‐positive. In 16% of AK , 80% of BD , 18% of SCC and 13% of BKL strong p16 staining was observed. BKL , AK and SCC showed focal and patchy staining, BD showed diffuse pattern with strong staining of all atypical cells. Sparing of the basal layer was predominantly seen in BD . No control specimen showed p16‐overexpression. Conclusions p16 immunostaining shows a characteristic pattern in BD , but not in AK , SCC and BKL . It appears useful in recognizing BD , but not in differentiating between other keratinocytic neoplasia.