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Expression of embryonic stem cell markers in pyogenic granuloma
Author(s) -
Blackwell Max G.,
Itinteang Tinte,
Chibnall Alice M.,
Davis Paul F.,
Tan Swee T.
Publication year - 2016
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/cup.12786
Subject(s) - homeobox protein nanog , sox2 , biology , population , stem cell , embryonic stem cell , pathology , in situ hybridization , stem cell marker , immunohistochemistry , microbiology and biotechnology , immunology , induced pluripotent stem cell , medicine , gene expression , genetics , environmental health , gene
Background Recent description of hemangioblastic blood islands within pyogenic granuloma ( PG ) has led us to investigate the expression of embryonic stem cell (ESC) markers in this tumor. Methods In this study we examined the expression of ESC markers, OCT4 , SOX2 , STAT3 and NANOG in PG samples from 11 patients, by immunohistochemical ( IHC ) staining, NanoString analysis and in situ hybridization ( ISH ). Results IHC staining demonstrated the expression of pSTAT3 , OCT4 , SOX2 and NANOG by the endothelium of the microvessels in PG whilst pSTAT3 , SOX2 and NANOG were also expressed by cells in the interstitium, outside of the microvessels. NanoString and ISH analysis showed mRNA expression for STAT3 , OCT4 and NANOG in PG . Conclusions The expression of the ESC markers, OCT4 , SOX2 , pSTAT3 and NANOG , suggests the endothelium of PG displays a primitive phenotype. Cells in the interstitium expressing pSTAT3 , SOX2 and NANOG may represent a more downstream derivative of the primitive endothelium, or a separate population. The primitive nature of the endothelium and cells in the interstitium reveals novel insights into the biology of PG . To the best of our knowledge, this is the first demonstration of the expression of ESC markers in PG , implying the presence of a hematopoietic stem cell population.