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Desmoplastic melanoma: an updated immunohistochemical analysis of 40 cases with a proposal for an additional panel of stains for diagnosis
Author(s) -
Plaza Jose A.,
Bonneau Peter,
Prieto Victor,
Sangueza Martin,
Mackin Alexander,
Suster David,
Bacchi Carlos,
Estrozi Bruna,
Kazakov Dmitry,
Kacerovska Denisa,
Falconieri Giovanni,
Suster Saul
Publication year - 2016
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/cup.12654
Subject(s) - nestin , cd117 , pathology , immunohistochemistry , sox10 , melanoma , microphthalmia associated transcription factor , medicine , immunostaining , cd34 , biology , cancer research , stem cell , neural crest , microbiology and biotechnology , embryo , biochemistry , neural stem cell , tyrosinase , enzyme
Desmoplastic melanoma ( DM ) is histologically characterized by a proliferation of spindle melanocytes dispersed in a collagenous stroma that can be mistaken for a variety of neoplasms. The purpose of this study was to analyze 40 cases of DM with a comprehensive panel of immunohistochemical markers ( KBA .62, p16, Ezrin, WT ‐1, MITF ‐1, SOX ‐10, CD117 , SOX ‐2, nestin, PNL2 , p75, MART ‐1, gp100 and S100p) to obtain a more complete understanding of the potential use of these antibodies in the diagnosis of DM . We found that all cases of DM expressed p16, WT ‐1, SOX ‐10, nestin and S100p and 95% of cases expressed p75. There was variable expression with Ezrin, SOX ‐2, KBA .62, MART ‐1 and HMB ‐45. Most DMs did not express MITF ‐1, PNL2 and CD117 . Conditions that may enter in the histologic differential diagnosis of DM , including dermal scars, fibromatosis and dermatofibromas were also studied. Nearly all control cases also stained positive for p16 but were negative for WT1 , SOX10 , nestin, p75 and S‐100p, as well as for most of the other markers tested. We conclude that a panel of S‐100p, WT1 , SOX10 , p75 and nestin may constitute the optimal panel with the most sensitive and specific combination of immunostain available for the diagnosis of DM .