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Peritumoral D2‐40 Chalkley score independently predicts metastases and survival in patients with cutaneous malignant melanoma
Author(s) -
Pastushenko Ievgenia,
Vermeulen Peter B.,
VicenteArregui Sandra,
Van den Eynden Gert G.,
AlvarezAlegret Ramiro,
Querol Ignacio,
Rutten Annemie,
Carapeto Francisco J.,
Dirix Luc Y.,
Van Laere Steven
Publication year - 2015
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/cup.12571
Subject(s) - lymphangiogenesis , medicine , melanoma , pathology , angiogenesis , lymph node , oncology , sentinel lymph node , cd34 , cancer , metastasis , breast cancer , cancer research , biology , stem cell , genetics
Background Many observational studies investigated the prognostic significance of angiogenesis and lymphangiogenesis in patients with melanoma. However, the obtained results are rather contradictory, probably due to the lack of the consensus methodology. Methods To investigate the prognostic significance of angiogenesis and lymphangiogenesis‐related parameters in patients with melanoma, we performed a retrospective investigation following the consensus recommendations for angiogenesis and lymphangiogenesis quantification in solid tumors and reporting recommendations for tumor marker ( REMARK ) criteria for reporting the results. Blood and lymphatic vessel Chalkley scores, endothelial cell proliferation fractions and microvessel densities were quantified using a double immunostaining for endothelial marker CD34 or lymphendothelial marker D240 and the proliferation marker Ki‐67 in 196 patients with melanoma. These parameters were evaluated separately for peritumoral (PT) and intratumoral areas and were correlated with outcome. Results In multivariate analysis PT D240 Chalkley score was identified as a strongest predictor for sentinel lymph node metastases, non‐sentinel lymph node metastases, distant metastases, disease free survival and overall survival in patients with melanoma. Conclusions If additional studies corroborate our findings, we believe that the inclusion of PT D240 Chalkley counts to the routine pathology examination of melanoma samples would provide additional information for identifying high‐risk patients.