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Levels of the EMT ‐related protein S nail/ S lug are not correlated with p53/p63 in cutaneous squamouscell carcinoma
Author(s) -
Chen Hongxiang,
Takahara Masakazu,
Xie Lining,
Takeuchi Satoshi,
Tu Yating,
Nakahara Takeshi,
Uchi Hiroshi,
Moroi Yoichi,
Furue Masutaka
Publication year - 2013
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/cup.12142
Subject(s) - slug , snail , immunohistochemistry , staining , actinic keratosis , pathology , seborrheic keratosis , metastasis , epithelial–mesenchymal transition , cadherin , biology , cancer research , carcinoma , cancer , cell , medicine , basal cell , ecology , genetics
Background The contribution of the E‐cadherin transcriptional repressors Snail and Slug to invasion and metastasis has strengthened the evidence for the importance of epithelial‐mesenchymal transition ( EMT ) in carcinoma progression. However, to the best of our knowledge, no study has described the immunohistochemical staining of the EMT ‐related proteins Snail/Slug in skin tumors and the correlation between Snail/Slug and tumor suppressor p53/p63. Methods We performed immunohistological staining of Snail, Slug, E‐cadherin, p53 and p63 in 20 archived specimens each of seborrheic keratosis ( SK ), actinic keratosis ( AK ) and squamous cell carcinoma in situ ( SCCIS ), and 53 specimens of cutaneous squamous cell carcinomas ( SCC ). Fifteen normal skin ( NS ) specimens served as controls. Results Significant negative correlations were observed between Snail and E‐cadherin expression and between Slug and E‐cadherin expression (Snail: R 2 = 0.5432, p < 0.01; Slug: R 2 = 0.4666, p < 0.01). Conclusions The staining intensities of Snail and Slug are associated with decreased E‐cadherin staining in SCC and this may promote EMT . However, the staining intensities of p53 and p63 are not significantly correlated with the loss of E‐cadherin.