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EGFR and MYC gene copy number aberrations are more common in squamous cell carcinoma than keratoacanthoma: a FISH study
Author(s) -
Jacobs Melissa S.,
Persons Diane L.,
Fraga Garth R.
Publication year - 2013
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1111/cup.12117
Subject(s) - keratoacanthoma , biology , immunohistochemistry , cancer research , epidermal growth factor receptor , fluorescence in situ hybridization , microbiology and biotechnology , gene , pathology , cancer , basal cell , medicine , immunology , genetics , chromosome
Epidermal growth factor receptor ( EGFR ) and MYC genomic aberrations have been described in cutaneous squamous cell carcinoma ( SCC ) but have not been widely investigated in keratoacanthoma ( KA ). EGFR and MYC were evaluated by fluorescence in situ hybridization and immunohistochemistry in 8 verrucae, 19 involuting KA (IKA), 23 classic KA ( CKA ), 6 atypical KA (AKA) and 19 SCC . Increased EGFR gene copy number was seen in 9 of 23 CKA and 14 of 19 SCC (p = 0.03). Increased MYC gene copy number was observed in 7 of 23 CKA and 17 of 19 SCC (p = 0.0001). MYC gene amplification was more common in SCC than CKA (p = 0.005), while EGFR gene amplification was rare and not significant. MYC protein overexpression was identified in 6 of 23 CKA and 14 of 19 SCC (p = 0.005). There was no statistical difference in EGFR protein overexpression in SCC and CKA (p = 0.06). EGFR and MYC aberrations were rare in IKA. AKA showed EGFR and MYC anomalies at an incidence intermediate between CKA and SCC . EGFR and MYC gene copy number aberrations are more common in SCC than KA . The incidence of aberrations parallels the degree of cytologic atypia in KA .