Open AccessGenotyping HLA‐B*5801 for Allopurinol‐Induced Severe Cutaneous Adverse Reactions: An Accurate and Prompt Method
Author(s) -
Cheng Lin,
Zhang Lihua,
Gao Lichen,
Zhang Wei,
Chen Xiaoping,
Zhou HongHao
Publication year - 2015
Publication title -
clinical and translational science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.303
H-Index - 44
eISSN - 1752-8062
pISSN - 1752-8054
DOI - 10.1111/cts.12365
Subject(s) - genotyping , allopurinol , medicine , scars , predictive value , genotype , surgery , biology , genetics , gene
Abstract Objectives A new method of HLA‐B*5801 genotyping was compared with sequence‐based typing (SBT) to find an accurate and prompt method in genotyping HLA‐B*5801 . Methods Two groups of patients from allopurinol‐induced cutaneous adverse reactions (SCARs) and allopurinol‐tolerant were both genotyped with PG5801 kit and SBT method. The genotyping results of HLA‐B*5801 were compared between the two groups. Results The PG5801 detection kit results were 100% (79/79) in agreement with the SBT genotyping results for identifying the HLA‐B*5801 (+) patients. No false‐positive or false‐negative errors were found. The sensitivity, specificity, rate of adherence, positive predictive value (PPV) and negative predictive value (NPV) were 100%. Conclusion The potential fast screening method is an ideal tool to rule out the high‐risk allopurinol‐induced SCARs patients.