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Reconstitution of T and NK cells after haploidentical hematopoietic cell transplantation using αβ T cell–depleted grafts and the clinical implication of γδ T cells
Author(s) -
Park Meerim,
Im Ho Joon,
Lee YuJin,
Park Nuree,
Jang Seongsoo,
Kwon Seog Woon,
Park ChanJeoung,
Choi Eun Seok,
Koh Kyung Nam,
Seo Jong Jin
Publication year - 2018
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1111/ctr.13147
Subject(s) - medicine , cd8 , transplantation , cytotoxic t cell , immunology , interleukin 21 , t cell , haematopoiesis , hematopoietic stem cell transplantation , leukemia , andrology , immune system , stem cell , biology , in vitro , genetics , biochemistry
Abstract To investigate reconstitution of T and NK cells after αβ T lymphocyte–depleted haploidentical hematopoietic cell transplantation ( HHCT ) and the clinical implications of γδ T cells, we analyzed 50 pediatric patients who received 55 HHCT s using αβ T cell–depleted grafts. The number of CD 3+ T cells and CD 8+ T cells recovered rapidly and reached donor levels at days 180 and 60, respectively. Recovery of NK cells was rapid, and the median of NK cells at day 14 was comparable to the donor level. At day 14, median percentage of γδ T lymphocytes was 70.5%. After day 14, the percentage of γδ T cells gradually decreased, while the percentage of αβ T cells gradually increased. Patients with a low percentage (≤21%) of γδ T cells at day 30 had significantly higher incidence of cytomegalovirus ( CMV ) reactivation compared to patients with a high percentage (>70%) of γδ T cells ( P < .01). In patients with acute leukemia, patients with high percentage of γδ T cells at day 30 showed significantly higher relapse‐free survival compared to those with low percentage of γδ T cells ( P = .02). Data suggest that early recovery of γδ T cells decreases the risk of CMV reactivation and leukemia relapse.