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Mast cell phenotypes in the allograft after lung transplantation
Author(s) -
Banga Amit,
Han Yingchun,
Wang Xiaofeng,
Hsieh Fred H.
Publication year - 2016
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1111/ctr.12758
Subject(s) - medicine , tryptase , chymase , lung transplantation , lung , transplantation , phenotype , mast cell , gastroenterology , pathology , biopsy , parenchyma , immunology , gene , biochemistry , chemistry
Background The burden of mast cell ( MC ) infiltration and their phenotypes, MC ‐tryptase ( MC T ) and MC ‐tryptase/chymase ( MC TC ), after lung transplantation ( LT ) has not been evaluated in human studies. Methods We reviewed 20 transbronchial lung biopsy ( TBLB ) specimen from patients with early normal allograft (<6 months post‐ LT , n=5), late normal allograft (>6 months, n=5), A2 or worse acute cellular rejection ( ACR , n=5), and chronic lung allograft dysfunction ( CLAD , n=5). Slides were immunostained for tryptase and chymase. Total MC , MC T , MC TC and MC TC to‐ MC T ratio were compared between the four groups using a generalized linear mixed model. Results Irrespective of clinicopathologic diagnosis, MC burden tends to increase with time ( r 2 =.56, P =.009). MC TC phenotype was significantly increased in the CLAD group (8.2±4.9 cells per HPF ) in comparison with the other three groups (early normal: 1.6±1.7, P =.0026; late normal: 2.5±2.3, P =.048; ACR : 2.7±3.5, P =.021). Further, the ratio of MC TC to MC T was significantly increased in CLAD group as compared to the other three groups ( P <.001 for all comparisons). Conclusions The burden of MC may increase in the allograft as function of time. Patients with CLAD have an increased relative and absolute burden of MC TC phenotype MC . Future studies are needed to confirm these findings and evaluate the potential pathologic role of MC TC in allograft dysfunction.