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The defects of cholangiocyte primary cilia in patients with graft cholangiopathies
Author(s) -
Lu Hongwei,
Dong Jiahong,
Li Chonghui,
Yu Qiang,
Tang Wei
Publication year - 2014
Publication title -
clinical transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 76
eISSN - 1399-0012
pISSN - 0902-0063
DOI - 10.1111/ctr.12419
Subject(s) - cholangiocyte , cilium , medicine , intrahepatic bile ducts , biliary tract , liver transplantation , pathology , western blot , ciliopathy , bile duct diseases , transplantation , extrahepatic bile ducts , gastroenterology , bile duct , phenotype , biology , microbiology and biotechnology , biochemistry , gene
Purpose To observe the morphologic changes in intrahepatic bile ducts and the defects of cholangiocyte primary cilia in patients with graft cholangiopathies. Methods Four patients who were diagnosed as graft cholangiopathies and underwent retransplantation were chosen as the study group; another four patients who underwent liver transplantation during the same period and recovered normally six months after the operation were the control group. The serum levels of biochemical indicators were measured, the morphologic changes in intrahepatic bile ducts and cholangiocyte primary cilia were observed, and the ciliary marker (α‐tubulin) and membrane proteins (polycystin‐1, TPPV 4) were detected by immunofluorescence analysis and W estern blot. Results In the study group, biliary structures were vague and some bile ducts disappeared in portal areas; some epithelial cells were lost; lots of collagen was deposited and many phlogocytes infiltrated; microliths were found in some ductal lumens; partial biliary epithelial cells were necrosed; primary cilia and microvilli disappeared. In the control group, the structures of intrahepatic bile ducts and biliary epithelial cells were integrated and the primary cilia were present. Conclusions The morphologic changes in biliary epithelial cells and the defects of cholangiocyte primary cilia have a close correlation with graft cholangiopathies in liver transplantation.