Open AccessSystemic oxidative stress in children with cystic fibrosis with bacterial infection including Pseudomonas Aeruginosa
Author(s) -
Bennemann Gabriela Datsch,
Moreira Emilia Addison Machado,
Pereira Leticia Cristina Radin,
Freitas Maiara Brusco,
Oliveira Diane,
Ventura Julia Carvalho,
Parisotto Eduardo Benedetti,
Moreno Yara Maria Franco,
Trindade Erasmo Benicio Santos Moraes,
Barbosa Eliana,
Ludwig Neto Norberto,
Wilhelm Filho Danilo
Publication year - 2022
Publication title -
the clinical respiratory journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.789
H-Index - 33
eISSN - 1752-699X
pISSN - 1752-6981
DOI - 10.1111/crj.13513
Subject(s) - tbars , cystic fibrosis , pseudomonas aeruginosa , oxidative stress , medicine , catalase , bacteriology , glutathione , thiobarbituric acid , microbiology and biotechnology , gastroenterology , biomarker , lipid peroxidation , bacteria , chemistry , biochemistry , biology , enzyme , genetics
Oxidative stress (OS) occurs in cystic fibrosis (CF). Objective The objective of this work is to evaluate the influence of bacterial infection on biomarkers of OS (catalase [CAT], glutathione peroxidade [GPx], reduced glutathione [GSH]), markers of oxidative damage (protein carbonyls [PC], thiobarbituric acid reactive substances [TBARS]), together with the nutritional status and lung function in children with CF. Methods Cross‐sectional study including CF group (CFG, n = 55) and control group (CG, n = 31), median age: 3.89 and 4.62 years, respectively. CFG was distributed into CFG negative bacteriology (CFGB−, n = 27) or CFG positive bacteriology (CFGB+, n = 28), and CFG negative Pseudomonas aeruginosa (CFGPa−, n = 36) or CFG positive Pseudomonas aeruginosa (CFGPa+, n = 19). Results Compared with CG, CFG ( P = .034) and CFGB+ ( P = .042) had lower body mass index‐for‐age z ‐score; forced expiratory volume in the first second was lower in CFGB+ and CFGPa+ (both P < .001). After adjusting for confounders and compared with CG: CFG showed higher TBARS ( P ≤ .001) and PC ( P = .048), and lower CAT ( P = .004) and GPx ( P = .003); the increase in PC levels was observed in CFGB+ ( P = .011) and CFGPa+ ( P = .001) but not in CFGB− ( P = .510) and CFGPa− ( P = .460). Conclusions These results indicate a systemic OS in children with CF. The presence of bacterial infection particularly Pseudomonas aeruginosa seems to be determinant to exacerbate the oxidative damage to proteins, in which PC may be a useful biomarker of OS in CF.