Open AccessAnti‐tumour effects of Keratin 6A in lung adenocarcinoma
Author(s) -
Xiao Jian,
Kuang Xiao,
Dai Longxia,
Zhang Lihai,
He Bixiu
Publication year - 2020
Publication title -
the clinical respiratory journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.789
H-Index - 33
eISSN - 1752-699X
pISSN - 1752-6981
DOI - 10.1111/crj.13182
Subject(s) - adenocarcinoma , keratin 6a , western blot , immunohistochemistry , lung , keratin , cancer research , medicine , pathology , gentamicin protection assay , adenocarcinoma of the lung , protein expression , lung cancer , cell , biology , cancer , metastasis , intermediate filament , gene , biochemistry , cytoskeleton , genetics
Background To examine the effects of Keratin 6A (KRT6A) protein on the proliferation, migration and invasion abilities of lung adenocarcinoma cells, and to analyse the relationship between the expression level of KRT6A protein and the survival prognosis of lung adenocarcinoma patients. Methods Western Blot was used to detect the expression of KRT6A protein in lung adenocarcinoma cell lines. CCK‐8 experiment and colony formation assays were performed to detect the proliferation ability. Wound healing assay and transwell migration assay were conducted to detect the migration ability. Transwell invasion assay was conducted to detect the invasion ability. Immunohistochemistry was used to detect the expression of KRT6A protein in lung adenocarcinoma tissues. Results We first found that the expression of KRT6A protein in lung adenocarcinoma cell lines was low. After overexpressed KRT6A protein in lung adenocarcinoma cells, we then found that KRT6A protein could not only inhibit the proliferation ability of lung adenocarcinoma cells but also inhibit them migration and invasion abilities. In addition, we also found that there had obvious difference in the expression of KRT6A protein in between patients. And through further analysis, we finally discovered that high expression of KRT6A protein was related to favourable prognosis in lung adenocarcinoma patients. Conclusions KRT6A protein inhibits the proliferation, migration and invasion abilities of lung adenocarcinoma cells, and high expression of KRT6A protein is a predictor of good prognosis in patients with lung adenocarcinoma.