Open AccessOxidative stress and inflamatory plasma biomarkers in respiratory syncytial virus bronchiolitis
Author(s) -
MorenoSolís Gloria,
dela TorreAguilar María José,
TorresBorrego Javier,
LlorenteCantarero Francisco Jesus,
FernándezGutiérrez Fernando,
GilCampos Mercedes,
TúnezFiñana Isaac,
PérezNavero Juan Luis
Publication year - 2017
Publication title -
the clinical respiratory journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.789
H-Index - 33
eISSN - 1752-699X
pISSN - 1752-6981
DOI - 10.1111/crj.12425
Subject(s) - medicine , bronchiolitis , oxidative stress , respiratory system , virus , microbiology and biotechnology , virology , biology
Oxidative stress (OS) plays a crucial role in the pathogenesis of inflammatory lung diseases. Objectives (i) We determined whether acute bronchiolitis (AB) caused by respiratory syncytial virus (RSV) induced OS; (ii) assessed whether OS biomarkers correlated with the severity of RSV‐AB; and (iii) studied whether the levels of interleukins are associated with OS biomarkers. Methods We performed an observational study by comparing healthy infants (Group 1) with RSV‐AB infants, classified as Group 2 (pulse oximetry (SpO 2 ) >93%), and Group 3 (SpO 2 ≤ 92%), which needed oxygen therapy. Blood samples were collected to determine the levels of lipid peroxidation (LPO) products (LPO), total glutathione (TG), oxidised glutathione (GSSG), reduced glutathione (GSH), glutathione peroxidase (GPx), interleukins (ILs) IL‐10, IL‐6, IL‐8, interferon‐gamma (IFNγ), tumour necrosis factor‐alpha (TNFα) and macrophage inflammatory proteins (MIP α and MIP β). Results Forty‐six RSV‐AB infants (47% needed oxygen therapy) and 27 healthy infants were included. The GSH/GSSG ratio was lower in RSV‐AB infants than in Group 1 ( P <0.001). GSSG and GPx were significantly higher in Group 3. GSSG predicted the need for oxygen therapy with an optimal cut‐off point of 15 µM/g for haemoglobin. The GSH/GSSG ratio negatively correlated with IL‐6 ( P : 0.014), IL‐8 (P : 0.014) and IL‐10 ( P : 0.033). Group 3 exhibited a direct correlation between GPx and IL‐10 levels ( P : 0.024) and between LPO and MIP β ( P : 0.003). Conclusions RSV induced OS in AB. An increase in GSSG correlated with the disease severity in the infants. OS may contribute to the pathogenesis of RSV‐AB.