Open AccessUp‐regulated integrinα4β1 on systemic lymphocytes and serum IL ‐17 A in interstitial pneumonia
Author(s) -
Taooka Yasuyuki,
Ohe Miki,
Tada Mitsuhiro,
Sutani Akihisa,
Isobe Takeshi
Publication year - 2016
Publication title -
the clinical respiratory journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.789
H-Index - 33
eISSN - 1752-699X
pISSN - 1752-6981
DOI - 10.1111/crj.12277
Subject(s) - medicine , integrin , immunology , integrin alpha m , interleukin , lung , interleukin 6 , pathology , receptor , inflammation , cytokine , immune system
Background and Aims In interstitial pneumonia ( IP ), lymphocytes play an important role in lung injury and the involvement of integrinα4β1 on leukocytes has previously been reported in animal models. Although the integrinα4β1 expression level is known to be up‐regulated by inflammatory cytokines, the involvement of interleukin ( IL )‐17 A is unclear. The purpose of this study is to address the possible involvement of integrinα4β1 on circulating lymphocytes and its correlation with serum IL ‐17 A in interstitial lung diseases ( ILD s). Methods We measured the expression levels of integrinα4β1 on peripheral lymphocytes and the serum concentration of IL ‐17 A and IL ‐23 in subjects with ILD s ( n = 27; 14 males and 13 females, 66.7 ± 7.8 years old) and control subjects ( n = 10; 5 males and 5 females, 66.6 ± 4.6 years old). Results Recombinant IL ‐17 A up‐regulated expression levels of integrinα4β1 on healthy human lymphocytes in an in vitro experiment. Expression levels of integrinα4β1 were significantly higher in those with acute hypersensitivity pneumonitis ( HP ) and non‐specific IP ( NSIP ) compared with control. Serum IL ‐17 A concentration was also significantly increased in acute HP and NSIP subjects compared with control. And IL ‐17 A concentration positively correlated with integrinα4β1 expression level ( P < 0.05). Serum IL ‐23 was below the minimal detectable level in all subjects. Conclusions These findings suggest that up‐regulated levels of integrinα4β1 on systemic lymphocytes and elevated serum IL ‐17 A might be involved in the extravasation of lymphocytes in IP .