Open AccessEvaluation of our self‐designed nanometer silicon membrane sandwich cup system for diagnosing tuberculosis
Author(s) -
Peng Jun,
Liu Wenen,
Li HongLing,
Liu QingXia,
Liang XiangHui,
Hu Ke,
Jian ZiJuan,
Li YanBing,
Peng WanChan
Publication year - 2016
Publication title -
the clinical respiratory journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.789
H-Index - 33
eISSN - 1752-699X
pISSN - 1752-6981
DOI - 10.1111/crj.12273
Subject(s) - acid fast , sputum , medicine , bacilli , nanometre , tuberculosis , staining , silicon , microporous material , biomedical engineering , microbiology and biotechnology , nuclear medicine , pathology , materials science , bacteria , biology , composite material , optoelectronics , genetics
Background and Aims A nanometer silicon membrane sandwich cup system was self‐designed. It could concentrate the bacilli via 0.45‐μm microporous filter membrane and semi‐automate the acid‐fast bacilli ( AFB ) by a bacteria‐staining machine. The aim of the study was to assess the clinical value of our self‐designed system for diagnosing tuberculosis ( TB ). Methods A total of 1993 sputum specimens obtained from patients with confirmed or suspected TB were subjected to direct or concentrated specimens smear at X iang Y a H ospital, C entral S outh U niversity between M ay 2012 and F ebruary 2013. In addition, all the specimens were also inoculated into L owenstein– J ensen ( L ‐ J ) media, and culture results were considered as the gold standard for calculating sensitivity and specificity. Results Compared with direct smear examination, an increased density of red stained bacilli was observed in the self‐designed nanometer silicon membrane sandwich cup analysis under the microscope. The positive rate of the self‐designed analysis was significantly higher than that of direct AFB smear [10.9% (217/1993) vs 6.2% (123/1993), P < 0.05]. The sensitivity of the self‐designed system increased (97.3% vs 55.2%, P < 0.05) without a loss of specificity (100% vs 100%) for identifying positive TB cases compared with the direct smear method. Conclusion The self‐designed nanometer silicon membrane sandwich cup and semi‐automatic bacteria‐staining machine could more efficiently and rapidly detect the AFB in respiratory specimens than direct microscopy. This is a novel and safe examination, and it may replace direct smear examination for the diagnosis of patients with TB .