Open AccessCytokine response to the 6‐min walk test in individuals with different degrees of COPD
Author(s) -
Dorneles Gilson Pires,
Vianna Priscila,
Del Duca Lima Diego,
Galant Lucas,
Dias Alexandre Simões,
Chies José Artur Bogo,
Monteiro Mariane Borba,
Peres Alessandra
Publication year - 2016
Publication title -
the clinical respiratory journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.789
H-Index - 33
eISSN - 1752-699X
pISSN - 1752-6981
DOI - 10.1111/crj.12221
Subject(s) - medicine , copd , cytokine , immune system , immunology , flow cytometry , interleukin 6 , gastroenterology , physical therapy
Background and Aim Physical exercise is a key part of rehabilitation programs in chronic obstructive pulmonary disease ( COPD ) patients, although it could modulates immune system responses by altering the cytokine profile of such individuals. Furthermore, the degree of severity of COPD could influence the inflammatory response induced by exercise. To evaluate the cytokine profile of individuals with different degrees of COPD in response to a 6‐min walk test (6 MWT ). Methods Forty‐one patients with COPD were classified according to the severity of the disease by G lobal I nitiative for C hronic O bstructive L ung D isease method: moderate = 14 individuals; severe = 14 individuals; very severe = 13 individuals. Blood sample collection was performed in the subjects pre and post a 6 MWT . Cytokine plasma levels were analyzed to determine the cytokine profile using a C ytometric B ead A rray technique ( B ecton D ickinson, San Jose, CA , USA ) assay in flow cytometry. Results A significant difference was observed in the interleukin ( IL )‐6 levels after test between very severe and severe groups ( P = 0.036). Also, lower levels of IL ‐4 were observed in the severe group compared with the very severe and the moderate groups in the pretest ( P = 0.029; P = 0.003, respectively), and different values between the moderate and severe groups in the post‐test ( P = 0.044). A significant time pre‐post effect was found in the IL ‐4 levels on the very severe group ( P = 0.046). Conclusion After the 6 MWT , a discrete inflammatory response was observed in COPD patients, independent of the degree of severity. The results concerning IL ‐4 and IL ‐6 levels can be indicative of an attempt to control inflammation after the 6 MWT in COPD patients.