Open AccessImmune repertoire analysis of normal Chinese donors at different ages
Author(s) -
Song Cailing,
Pan Wenjing,
Brown Brittany,
Tang Congli,
Huang Yunqi,
Chen Houao,
Peng Nan,
Wang Zhe,
Weber Daniel,
ByrneSteele Miranda,
Wu Haijing,
Liu Hongna,
Deng Yan,
He gyue,
Li Song
Publication year - 2022
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.13311
Subject(s) - repertoire , t cell receptor , complementarity determining region , immune system , biology , antibody , immunology , antibody repertoire , receptor , gene , immunoglobulin light chain , genetics , t cell , acoustics , physics
Abstract Objectives This study investigated the characteristics of the immune repertoire in normal Chinese individuals of different ages. Materials and Methods In this study, all seven receptor chains from both B and T cells in peripheral blood of 16 normal Chinese individuals from two age groups were analyzed using high‐throughput sequencing and dimer‐avoided multiplex PCR amplification. Normal in this study is defined as no chronic, infectious or autoimmune disease within 6 months prior to blood draw. Results We found that compared with the younger group, the clonal expression of T‐cell receptor repertoire increased in the older group, while diversity decreased. In addition, we found that the T‐cell receptor repertoire was more significantly affected by age than the B‐cell receptor repertoire, including significant differences in the use of the unique TCR‐alpha and TCR‐beta V‐J gene combinations, in the two groups of normal participants. We further analyzed the degree of complementarity determining region 3 sequence sharing between the two groups, and found shared TCR‐alpha, TCR‐gamma, immunoglobulin‐kappa and immunoglobulin‐lambda chain complementarity determining region 3 sequences in all subjects. Conclusion Taken together, our study gives us a better understanding of the immune repertoire of different normal Chinese people, and these results can be applied to the treatment of age‐related diseases. Immune repertoire analysis also allows us to observe participant's wellness, aiding in early‐stage diagnosis.