Open AccessDental pulp stem cells‐based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo
Author(s) -
Luo Lihua,
Xing Zhenjie,
Liao Xiangyan,
Li Yejian,
Luo Yu,
Ai Yilong,
He Yan,
Ye Qingsong
Publication year - 2022
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.13293
Subject(s) - in vivo , dental pulp stem cells , regeneration (biology) , oviduct , angiogenesis , flow cytometry , in vitro , immunohistochemistry , microbiology and biotechnology , biology , stem cell , andrology , pathology , medicine , immunology , cancer research , biochemistry
Objectives As a result of the current limitation of therapeutic strategies, the repair and regeneration of oviduct injuries required an alternative treatment. We present a novel approach to treat oviduct injuries through a dental pulp stem cells (DPSCs)‐based therapy. Materials and Methods In vitro and in vivo models have been established. Immunofluorescence staining, flow cytometry and enzyme‐linked immunosorbent assay (ELISA) analysis were used to investigate the features and angiogenic properties of DPSCs, as well as their impact on macrophages, in vitro. For the in vivo experiment with female SD rat model, immunohistochemical staining and ELISA analysis were used to assess the effects of DPSCs on the repair and regeneration of damaged oviducts. Results The present data showed that intraperitoneal injection of DPSCs reduced the expression of IL‐6 and TNF‐α to inhibit the immunoreaction in injured sites, as well as increased the expression of VEGF to promote the in situ formation of vessel‐like structures, thus the repair and recovery process could be initiated. Conclusions We concluded that DPSCs‐based therapy could be a novel potential technique for restoring the structure and function of damaged oviduct by enhancing immuno‐regulated effect and promoting angiogenic property.