Open AccessDifferences in the stemness characteristics and molecular markers of distinct human oral tissue neural crest‐derived multilineage cells
Author(s) -
Abe Shigehiro,
Kaida Atsushi,
Kanemaru Kazunori,
Nakazato Keiichiro,
Yokomizo Naoko,
Kobayashi Yutaka,
Miura Masahiko,
Miki Toshio,
Hidai Chiaki,
Kitano Hisataka,
Yoda Tetsuya
Publication year - 2022
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.13286
Subject(s) - neural crest , biology , stem cell , dental papilla , microbiology and biotechnology , progenitor cell , stem cell marker , cd24 , cellular differentiation , oral mucosa , pathology , anatomy , pulp (tooth) , genetics , odontoblast , gene , cancer stem cell , medicine , embryo
Objectives Although multilineage cells derived from oral tissues, especially the dental pulp, apical papilla, periodontal ligament, and oral mucosa, have neural crest‐derived stem cell (NCSC)‐like properties, the differences in the characteristics of these progenitor cell compartments remain unknown. The current study aimed to elucidate these differences. Material and methods Sphere‐forming apical papilla‐derived cells (APDCs), periodontal ligament‐derived cells (PDLDCs), and oral mucosa stroma‐derived cells (OMSDCs) from the same individuals were isolated from impacted developing teeth. All sphere‐forming cells were characterized through biological analyses of stem cells. Results All sphere‐forming cells expressed neural crest‐related markers. The expression of certain tissue‐specific markers such as CD24 and CD56 (NCAM1) differed among tissue‐derived cells. Surprisingly, the expression of only CD24 and CD56 could be discriminated in human tissues. Although APDCs and PDLDCs exhibited greater mineralized cell differentiation than OMSDCs, they exhibited poorer differentiation into adipocytes in vitro . In immunocompromised mice, APDCs formed hard tissues better than PDLDCs and OMSDCs. Conclusions Although cells with NCSC‐like properties present the same phenotype, they differ in the expression of certain markers and differentiation abilities. This study is the first to demonstrate the differences in the differentiation ability and molecular markers among multilineage human APDCs, PDLDCs, and OMSDCs obtained from the same patients, and to identify tissue‐specific markers that distinguish tissues in the developing stage of the human tooth with immature apex.