Open AccessThe proliferative and multipotent epidermal progenitor cells for human skin reconstruction in vitro and in vivo
Author(s) -
Lim Jung Hwa,
Kim Dae Hun,
Noh Kyung Hee,
Jung ChoRok,
Kang Hyun Mi
Publication year - 2022
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.13284
Subject(s) - progenitor cell , microbiology and biotechnology , biology , multipotent stem cell , stem cell , regeneration (biology) , mesenchymal stem cell , in vivo , wound healing , epidermis (zoology) , progenitor , keratinocyte , dermis , cellular differentiation , in vitro , immunology , anatomy , biochemistry , gene
Objectives The skin exhibits tremendous regenerative potential, as different types of progenitor and stem cells regulate skin homeostasis and damage. However, in vitro primary keratinocytes present with several drawbacks, such as high donor variability, short lifespan, and limited donor tissue availability. Therefore, more stable primary keratinocytes are needed to generate multiple uniform in vitro and in vivo skin models. Results We identified epidermal progenitor cells from primary keratinocytes using Integrin beta 1 (ITGB1) an epidermal stem cell marker markedly decreased after senescence in vitro. Epidermal progenitor cells exhibited unlimited proliferation and the potential for multipotent differentiation capacity. Moreover, they could completely differentiate to form an organotypic skin model including conversed mesenchymal cells in the dermis and could mimic the morphologic and biochemical processes of human epidermis. We also discovered that proliferation and the multipotent differentiation capacity of these cells relied on ITGB1 expression. Eventually, we examined the in vitro and in vivo wound healing capacity of these epidermal progenitor cells. Conclusions Overall, the findings suggest that these stable and reproducible cells can differentiate into multiple lineages, including human skin models. They are a potentially powerful tool for studying skin regeneration, skin diseases, and are an alternative for in vivo experiments.