Open AccessGFI1 regulates chromatin state essential in human endothelial‐to‐haematopoietic transition
Author(s) -
Kang Baoqiang,
Zhang Tian,
Huang Ke,
Wang Tianyu,
Li Yuhang,
Mai Yuchan,
Li Jinbing,
Dang Shiying,
Zhang Zhishuai,
Huang Wenhao,
Wang Junwei,
Gao Minghui,
Wang Yi,
Pan Guangjin
Publication year - 2022
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.13244
Subject(s) - haematopoiesis , embryonic stem cell , biology , microbiology and biotechnology , chromatin , epigenetics , stem cell , progenitor cell , genetics , gene
Objectives During embryonic haematopoiesis, haematopoietic stem/progenitor cells (HSPCs) develop from hemogenic endothelial cells (HECs) though endothelial to haematopoietic transition (EHT). However, little is known about how EHT is regulated in human. Here, we report that GFI1 plays an essential role in enabling normal EHT during haematopoietic differentiation of human embryonic stem cells (hESCs). Results GFI1 deletion in hESCs leads to a complete EHT defect due to a closed chromatin state of hematopoietic genes in HECs. Mechanically, directly regulates important signaling pathways essential for the EHT such as PI3K signaling.etc. Conclutions Together, our findings reveal an essential role of GFI1 mediated epigenetic mechanism underlying human EHT during hematopoiesis.