Open AccessThe role and potential mechanism of p75NTR in mineralization via in vivo p75NTR knockout mice and in vitro ectomesenchymal stem cells
Author(s) -
Zhao Manzhu,
Wang Yingying,
Li Gang,
Li Jun,
Yang Kun,
Liu Chang,
Wen Xiujie,
Song Jinlin
Publication year - 2020
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12758
Subject(s) - bone sialoprotein , knockout mouse , osteopontin , chemistry , in vivo , in vitro , runx2 , osteocalcin , endocrinology , medicine , microbiology and biotechnology , alkaline phosphatase , biology , osteoblast , biochemistry , receptor , enzyme
Abstract Objective The aim of this study is to investigate the role and potential mechanism of p75NTR in mineralization in vivo using p75NTR‐knockout mice and in vitro using ectomesenchymal stem cells (EMSCs). Materials and methods Femur bone mass and daily incisor mineralization speed were assessed in an in vivo p75NTR‐knockout mouse model. The molecular signatures alkaline phosphatase (ALP), collagen type 1 (Col1), melanoma‐associated antigen (Mage)‐D1, bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN), distal‐less homeobox 1 (Dlx1) and Msh homeobox 1 (Msx1) were examined in vitro in EMSCs isolated from p75NTR +/+ and p75NTR ExIII−/− mice. Results p75NTR‐knockout mice were smaller in body size than heterozygous and wild‐type mice. Micro‐computed tomography and structural quantification showed that the osteogenic ability of p75NTR ExIII ‐knockout mice was significantly decreased compared with that of wild‐type mice ( P < .05). Weaker ALP and alizarin red staining and reduced expression of ALP, Col1, Runx2, BSP, OCN and OPN were also observed in p75NTR ExIII−/− EMSCs. Moreover, the distance between calcein fluorescence bands in p75NTR ExIII ‐knockout mice was significantly smaller than that in wild type and heterozygous mice ( P < .05), indicating the lower daily mineralization speed of incisors in p75NTR ExIII ‐knockout mice. Further investigation revealed a positive correlation between p75NTR and Mage‐D1, Dlx1, and Msx1. Conclusion p75NTR not only promotes osteogenic differentiation and tissue mineralization, but also shows a possible relationship with the circadian rhythm of dental hard tissue formation.