Open AccessThe role of Sprouty1 in the proliferation, differentiation and apoptosis of epidermal keratinocytes
Author(s) -
Wang Ping,
Zhou Yuan,
Yang JianQiang,
Landeck Lilla,
Min Min,
Chen XiBei,
Chen JiaQi,
Li Wei,
Cai SuiQing,
Zheng Min,
Man XiaoYong
Publication year - 2018
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12477
Subject(s) - epidermis (zoology) , keratinocyte , biology , apoptosis , cyclin d1 , microbiology and biotechnology , cell growth , cancer research , cell cycle , immunology , in vitro , anatomy , genetics
Objectives Sprouty ( SPRY ) 1 is one of the SPRY proteins that inhibits signalling from various growth factors pathways and has also been known as a tumour suppressor in various malignancies. However, no study elucidates the role of SPRY 1 in the skin. Our study was conducted to determine the function of SPRY 1 in human keratinocytes and the epidermis. Materials and methods In vitro primary cultured epidermal keratinocytes were used to investigate the proliferation, differentiation and apoptosis of these cells. We also established overexpression of SPRY 1 in vitro and K14‐ SPRY 1 transgenic mice. Results SPRY 1 was mainly located in the cytoplasm of the epidermal keratinocytes from the granular epidermal layer of the skin and cultured cells. Overexpressed SPRY 1 in keratinocytes resulted in up‐regulation of P21, P27 and down‐regulation of cyclin B1; decrease in MMP 3 and integrin α6. SPRY 1‐overexpressed primary keratinocytes exhibited a lower proliferation and migration capability and higher rates of apoptosis. Epidermis of SPRY 1‐ TG mice represented delayed wound healing. Proteomics analysis and GO enrichment showed DEP s of SPRY 1 TG mice epidermis is significantly enriched in immune‐ and inflammatory‐associated biological process. Conclusions In summary, SPRY 1 expression was inversely correlated with cell proliferation, migration and promote cell apoptosis of keratinocytes. SPRY 1 maybe a negative feedback regulator in normal human epidermal keratinocytes and cutaneous inflammatory responses. Our study raised the possibility that enhancing expression of SPRY 1 may have the potential to promote anti‐inflammatory effects.