Open AccessLong noncoding RNA LINC 00978 promotes cancer growth and acts as a diagnostic biomarker in gastric cancer
Author(s) -
Fu Min,
Huang Zhenhua,
Zang Xueyan,
Pan Lei,
Liang Wei,
Chen Jingyan,
Qian Hui,
Xu Wenrong,
Jiang Pengcheng,
Zhang Xu
Publication year - 2018
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12425
Subject(s) - gene knockdown , cancer research , biology , cancer , long non coding rna , metastasis , cell growth , cancer cell , apoptosis , microbiology and biotechnology , rna , gene , genetics
Abstract Objectives Long noncoding RNA s (lnc RNA s) play important roles in cancer development and progression. The deregulated expression of LINC 00978 has been reported in human cancers. However, the expression pattern and biological roles of LINC 00978 in gastric cancer ( GC ) remain unclear. In this study, we investigated the potential roles and clinical value of LINC 00978 in gastric cancer. Materials and methods QRT ‐ PCR was performed to investigate the expression of LINC 00978 in gastric cancer cell lines, tissues and serum samples. Cell counting, colony formation, transwell migration and matrigel invasion assays were performed to determine the effects of sh RNA ‐mediated knockdown of LINC 00978 on gastric cancer cell functions. In vivo tumour growth assay was also conducted. Flow cytometry, immunohistochemistry, western blot and qRT ‐ PCR were used for potential mechanism study. Results LINC 00978 expression level was elevated in GC tumour tissues, serum samples and cell lines. The expression level of LINC 00978 was significantly correlated with tumour size ( P = 0.02), lymphatic metastasis ( P = 0.009) and TNM stage ( P = 0.009). LINC 00978 knockdown inhibited the proliferation of GC cells by suppressing cell cycle progression and inducing apoptosis. LINC 00978 knockdown also inhibited the migration and invasion of GC cells. In addition, LINC 00978 knockdown inhibited the activation of TGF ‐β/ SMAD signalling pathway and the process of epithelial‐mesenchymal transition ( EMT ) in GC cells. Moreover, the in vivo tumorigenicity of LINC 00978 knockdown GC cells in mice was significantly decreased. Conclusions LINC 00978 promotes gastric cancer progression and may serve as a potential biomarker for GC .