Open AccessMesenchymal‐like stem cells in canine ovary show high differentiation potential
Author(s) -
Trindade Hill A. B.,
Therrien J.,
Garcia J. M.,
Smith L. C.
Publication year - 2017
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12391
Subject(s) - mesenchymal stem cell , homeobox protein nanog , stem cell , adipose tissue , adipogenesis , biology , microbiology and biotechnology , chondrogenesis , cellular differentiation , embryonic stem cell , endocrinology , induced pluripotent stem cell , biochemistry , gene
Abstract Objectives Recent studies have reported the existence of stem cells in ovarian tissue that show enhanced proliferative and differentiation potential compared to other adult tissues. Based on this evidence, we hypothesized that ovarian tissue contained mesenchymal‐like stem cells ( MSC ) that could be isolated using a novel rapid plastic adhesion technique. Materials and methods We established MSC lines derived from ovarian and adipose tissue based on their ability to rapidly adhere to plastic culture dishes in the first 3 hours after plating and studied their potentiality in terms of molecular markers and differentiation capacity. Results Morphological and kinetic properties of in vitro cultured ovarian MSC were similar to adipose‐derived MSC , and both reached senescence after similar passage numbers. Ovarian‐derived MSC expressed mesenchymal ( CD 90 and CD 44) but not haematopoietic markers ( CD 34 and CD 45), indicating similarity to adipose‐derived MSC . Moreover, ovarian‐derived MSC expressed NANOG , TERT , SOX 2, OCT 4 and showed extensive capacity to differentiate not only into adipogenic, osteogenic and chondrogenic tissue but also towards neurogenic and endodermal lineages and even precursors of primordial germ cells. Conclusion These results show for the first time the derivation of ovarian cells with the molecular properties of MSC as well as wide differentiation potential. Canine ovarian tissue is accessible, expandable, multipotent and has high plasticity, holding promise for applications in regenerative medicine.