Open AccessLow‐intensity pulsed ultrasound activates ERK 1/2 and PI 3K‐Akt signalling pathways and promotes the proliferation of human amnion‐derived mesenchymal stem cells
Author(s) -
Ling Li,
Wei Tianqin,
He Lianli,
Wang Yaping,
Wang Yan,
Feng Xiushan,
Zhang Wenqian,
Xiong Zhengai
Publication year - 2017
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12383
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , mapk/erk pathway , amnion , stem cell , cell growth , protein kinase b , cell cycle , low intensity pulsed ultrasound , cyclin b1 , cyclin d1 , chemistry , biology , signal transduction , cell , cyclin dependent kinase 1 , biochemistry , medicine , ultrasound , pregnancy , fetus , genetics , radiology , therapeutic ultrasound
Objectives This study was to investigate the effect and mechanism of low‐intensity pulsed ultrasound ( LIPUS ) on the proliferation of human amnion‐derived mesenchymal stem cells ( hAD ‐ MSC s). Methods Human amnion‐derived mesenchymal stem cells were isolated from the amnion of term placentas and identified by flow cytometry and differentiation culture. Proliferation of hAD ‐ MSC s was investigated by Cell Counting Kit‐8, cell cycle and EdU assays. Western blotting was used to determine the protein expression levels. Results Human amnion‐derived mesenchymal stem cells were successfully isolated from the amnion and identified as multipotent mesenchymal stem cells. Low‐intensity pulsed ultrasound promoted the proliferation of hAD ‐ MSC s. Cell cycle analysis showed that LIPUS promoted cells to enter S and G2/M phases from G0/G1 phase. Western blot results showed that LIPUS promoted the phosphorylation and activation of ERK 1/2 and Akt and significantly upregulated expression of cyclin D1, cyclin E1, cyclin A2 and cyclin B1. ERK 1/2 inhibitor (U0126) and PI 3K inhibitor ( LY 294002) significantly reduced LIPUS ‐induced phosphorylation of ERK 1/2 and Akt, respectively, which in turn reduced the LIPUS ‐induced proliferation of hAD ‐MSCs. Conclusions Low‐intensity pulsed ultrasound can promote the proliferation of hAD ‐ MSC s, and ERK 1/2 and PI 3K‐Akt signalling pathways may play important roles in this process.