Open AccessModulation of chondrocyte motility by tetrahedral DNA nanostructures
Author(s) -
Shi Sirong,
Lin Shiyu,
Shao Xiaoru,
Li Qianshun,
Tao Zhang,
Lin Yunfeng
Publication year - 2017
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12368
Subject(s) - rhoa , vinculin , chondrocyte , motility , chemistry , microbiology and biotechnology , polyethylenimine , blot , biophysics , dna , transfection , biochemistry , cell adhesion , cell , signal transduction , biology , gene , in vitro
Abstract Objectives Contemporarily, a highly increasing attention was paid to nanoconstructs, particularly DNA nanostructures possessing precise organization, functional manipulation, biocompatibility and biodegradability. Amongst these DNA nanomaterials, tetrahedral DNA nanostructures ( TDN s) are a significantly ideal bionanomaterials with focusing on the property that can be internalized into cytoplasm in the absence of transfection. Therefore, the focus of this study was on investigating the influence of TDN s on the chondrocytes locomotion. Materials and methods Tetrahedral DNA nanostructures was confirmed by 6% polyacrylamide gel electrophoresis ( PAGE ) and dynamic light scattering ( DLS ). Subsequently, the effect of TDN s on chondrocyte locomotion was investigated by real‐time cell analysis ( RTCA ) and wound healing assay. The variation of relevant genes and proteins was detected by quantitative polymerase chain reaction ( qPCR ), western blotting and immunofluorescence respectively. Results We demonstrated that tetrahedral DNA nanostructures have positive influence on chondrocytes locomotion and promoted the expression of RhoA , ROCK 2 and vinculin . Additionally, upon exposure to TDN s with the concentration of 250 nmol L −1 , the chondrocytes were showed the highest motility via both RTCA and wound healing assay. Meanwhile, the mRNA and protein expression of RhoA, ROCK 2 and vinculin were also significantly enhanced with the same concentration. Conclusions It can be concluded that the TDN s with the optimal concentration of 250 nmol L −1 could extremely promoted the chondrocytes locomotion through facilitating the expression of RhoA , ROCK 2 and vinculin . These results seemed to reveal that this special three‐dimensional DNA tetrahedral nanostructures may be applied to cartilage repair and treatment in the future.