Open AccessLidocaine inhibits the proliferation of lung cancer by regulating the expression of GOLT 1A
Author(s) -
Zhang Lei,
Hu Rong,
Cheng Yanyong,
Wu Xiaoyang,
Xi Siwei,
Sun Yu,
Jiang Hong
Publication year - 2017
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12364
Subject(s) - lidocaine , cell growth , a549 cell , apoptosis , western blot , cell cycle , downregulation and upregulation , bromodeoxyuridine , lung cancer , chemistry , cancer research , pharmacology , biology , microbiology and biotechnology , medicine , pathology , anesthesia , biochemistry , gene
Objectives Lidocaine is the most commonly used local anaesthetic in clinical and can inhibit proliferation, suppress invasion and migration and induce apoptosis in human lung adenocarcinoma ( LAD ) cells. However, its specific downstream molecular mechanism is unclear. Materials and methods LAD cell lines, A549 and H1299 cells, were treated with lidocaine. The proliferation was evaluated by the methylthiazolyldiphenyl‐tetrazolium bromide ( MTT ) and bromodeoxyuridine (BrdU) assay. The expression level of related proteins was detected by real‐time quantitative PCR ( qPCR ) and Western blot assay. Results The results indicated that lidocaine dose‐dependently suppressed the proliferation of A549 and H1299 cells. In the LAD patients’ samples, GOLT 1A was upregulated and involved in the poor prognosis and higher grade malignancy. Additionally, GOLT 1A mediates the function of lidocaine on repressing proliferation by regulating the cell cycle in A549 cells. Conclusions Our findings suggest that lidocaine downregulates the GOLT 1A expression to repress the proliferation of lung cancer cells.