Open AccessOsteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides
Author(s) -
Lukasova Vera,
Buzgo Matej,
Sovkova Vera,
Dankova Jana,
Rampichova Michala,
Amler Evzen
Publication year - 2017
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12357
Subject(s) - osteocalcin , mesenchymal stem cell , chemistry , bone morphogenetic protein 2 , peptide , bone morphogenetic protein , microbiology and biotechnology , immunostaining , type i collagen , biochemistry , biology , in vitro , immunohistochemistry , immunology , alkaline phosphatase , gene , endocrinology , enzyme
Objectives Bioactive peptides derived from receptor binding motifs of native proteins are a potent source of bioactive molecules that can induce signalling pathways. These peptides could substitute for osteogenesis promoting supplements. The work presented here compares three kinds of bioactive peptides derived from collagen III , bone morphogenetic protein 7 ( BMP ‐7) and BMP ‐2 with their potential osteogenic activity on the model of porcine mesenchymal stem cells ( pMSC s). Materials and methods pMSC s were cultured on electrospun polycaprolactone nanofibrous scaffolds with different concentrations of the bioactive peptides without addition of any osteogenic supplement. Analysis of pMSC s cultures included measurement of the metabolic activity and proliferation, immunofluorescence staining and also qPCR . Results Results showed no detrimental effect of the bioactive peptides to cultured pMSC s. Based on qPCR analysis, the bioactive peptides are specific for osteogenic differentiation with no detectable expression of collagen II . Our results further indicate that peptide derived from BMP ‐2 protein promoted the expression of mRNA for osteocalcin ( OCN ) and collagen I significantly compared to control groups and also supported deposition of OCN as observed by immunostaining method. Conclusion The data suggest that bioactive peptide with an amino acid sequence of KIPKASSVPTELSAISTLYL derived from BMP ‐2 protein was the most potent for triggering osteogenic differentiation of pMSC s.