Open AccessmiR‐1297 regulates neural stem cell differentiation and viability through controlling Hes1 expression
Author(s) -
Zheng Jiaolin,
Yi Dan,
Shi Xiaodong,
Shi Huaizhang
Publication year - 2017
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12347
Subject(s) - hes1 , microbiology and biotechnology , biology , neurosphere , microrna , neural stem cell , viability assay , cellular differentiation , western blot , stem cell , cell , signal transduction , notch signaling pathway , genetics , gene , adult stem cell
Objectives Neural stem cells ( NSC s) are self‐renewing, undifferentiated and multipotent precursors that can generate neuronal and glial lineages. Micro RNA s (mi RNA s) are small non‐coding RNA s that act crucial roles in cell proliferation, differentiation and migration. However, the role of miR‐1297 in the development of NSC s is still unknown. Materials and methods Primary NSC s were isolated from rat's embryos. The expression of miR‐1297 and Hes1 were measured by qRT ‐ PCR . Western blot was performed to detect the protein expression of Hes1, β‐tubulin‐ III and GFAP . Results We showed that miR‐1297 expression was upregulated during NSC differentiation, while the expression of Hes1 was decreased during NSC differentiation. Elevated expression of miR‐1297 promoted the NSC s viability and increased the formation of NSC s to neurospheres. Ecoptic expression of miR‐1297 promoted β‐tubulin‐ III expression in the NSC s. Overexpression of miR‐1297 decreased GFAP expression in the NSC s. Furthermore, we demonstrated that miR‐1297 regulated NSC s viability and differentiation by directly targeting Hes1. Overexpression of miR‐1297 suppressed Hes1 expression in the NSC s. Conclusions These results suggested that miR‐1297 played an important role in NSC s viability and differentiation through inhibiting Hes1 expression.