Open AccessAnalysis of gene expression profiles between apical papilla tissues, stem cells from apical papilla and cell sheet to identify the key modulators in MSC s niche
Author(s) -
Diao Shu,
Lin Xiao,
Wang Liping,
Dong Rui,
Du Juan,
Yang Dongmei,
Fan Zhipeng
Publication year - 2017
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12337
Subject(s) - wnt signaling pathway , microbiology and biotechnology , biology , major duodenal papilla , gene , microarray analysis techniques , dermal papillae , apical cell , dental papilla , cell growth , gene expression , cell , anatomy , signal transduction , genetics , hair follicle , pathology , medicine , odontoblast , dentin
Objectives The microenvironmental niche plays the key role for maintaining the cell functions. The stem cells from apical papilla ( SCAP s) are important for tooth development and regeneration. However, there is limited knowledge about the key factors in niche for maintaining the function of SCAP s. In this study, we analyse the gene expression profiles between apical papilla tissues, SCAP s and SCAP s cell sheet to identify the key genes in SCAP s niche. Materials and methods Microarray assays and bioinformatic analysis were performed to screen the differential genes between apical papilla tissues and SCAP s, and SCAP s and SCAP s cell sheet. Recombinant human BMP 6 protein was used in SCAP s. Then CCK ‐8 assay, CFSE assay, alkaline phosphatase activity, alizarin red staining, quantitative calcium analysis and real‐time reverse transcriptase‐polymerase chain reaction were performed to investigate the cell proliferation and differentiation potentials of SCAP s. Results Microarray analysis found that 846 genes were up‐regulated and 1203 genes were down‐regulated in SCAP s compared with apical papilla tissues. While 240 genes were up‐regulated and 50 genes were down‐regulated in SCAP s compared to in SCAP s cell sheet. Moreover, only 31 gene expressions in apical papilla tissues were recovered in cell sheet compared with SCAP s. Bioinformatic analysis identified that TGF ‐β, WNT and MAPK signalling pathways may play an important role in SCAP s niche. Based on the analysis, we identified one key growth factor in niche, BMP 6, which could enhance the cell proliferation, the osteo/dentinogenic, neurogenic and angiogenic differentiation potentials of SCAP s. Conclusions Our results provided insight into the mechanisms of the microenvironmental niche which regulate the function of SCAP s, and identified the key candidate genes in niche to promote mesenchymal stem cells‐mediated dental tissue regeneration.