Open AccessThe effect of delta‐like 1 homologue on the proliferation and odontoblastic differentiation in human dental pulp stem cells
Author(s) -
Qi Shengcai,
Yan Yanhong,
Wen Yue,
Li Jialiang,
Wang Jing,
Chen Fubo,
Tang Xiaoshan,
Shang Guangwei,
Xu Yuanzhi,
Wang Raorao
Publication year - 2017
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12335
Subject(s) - dmp1 , dental pulp stem cells , mapk/erk pathway , chemistry , alkaline phosphatase , microbiology and biotechnology , dentin sialophosphoprotein , odontoblast , stem cell , dentin , biochemistry , biology , signal transduction , dentistry , enzyme , medicine , viral matrix protein , gene
This study aimed to investigate the functions of delta‐like homologue 1 ( DLK 1) in the proliferation and differentiation of human dental pulp stem cells ( hDPSC s). Methods Immunohistochemical analysis was used to determine the expression of alkaline phosphatase ( ALP ), dentin sialophosphoprotein ( DSPP ), DLK 1, NOTCH 1 and p‐ ERK 1/2 in the mouse first maxillary molar. Recombinant lentivirus was constructed to overexpress DLK 1 stably in hDPSC s. The cell viability and proliferation of hDPSC s were examined by CCK 8 and EdU incorporation assay respectively. The odontoblastic differentiation of hDPSC s was determined by detection of ALP ase activity assay, ALP and alizarin red staining and the expression of mineralization‐related genes including ALP , DSPP and dental matrix protein. The mRNA and protein levels of DLK 1 and p‐ ERK 1/2 protein expression were detected. ERK inhibitor was used to test the differentiation effect of DLK 1 on hDPSC s. Results Delta‐like homologue 1 was highly expressed on the odontoblasts and dental pulp cells on the first maxillary molar; the expression of p‐ ERK 1/2 is similar with the DLK 1 in the same process. The expression level of DLK 1 increased significantly after the odontoblastic induction of hDPSC s. DLK 1 overexpression increased the proliferation ability of hDPSC s and inhibited odontoblastic differentiation of hDPSC s. The protein level of p‐ ERK 1/2 significantly increased in hDPSC s/dlk1‐oe group. ERK signalling pathway inhibitor reversed the odontoblastic differentiation effects of DLK 1 on hDPSC s. Conclusions The proliferation of hDPSC s was promoted after DLK 1 overexpression. DLK 1 inhibited the odontoblastic differentiation of hDPSC s, which maybe through ERK signalling pathway.