Open AccessGene expression profiling of CD 8 + T cells induced by ovarian cancer cells suggests a possible mechanism for CD 8 + Treg cell production
Author(s) -
Wu Meng,
Lou Jianfang,
Zhang Shuping,
Chen Xian,
Huang Lei,
Sun Ruihong,
Huang Peijun,
Pan Shiyang,
Wang Fang
Publication year - 2016
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12294
Subject(s) - biology , gene expression , microbiology and biotechnology , cd8 , glycolysis , kegg , western blot , cell culture , gene expression profiling , gene , microarray analysis techniques , cancer research , transcriptome , immunology , metabolism , immune system , genetics , biochemistry
Objectives The aim of this study was to investigate a possible mechanism of CD 8 + regulatory T‐cell (Treg) production in an ovarian cancer ( OC ) microenvironment. Materials and methods Agilent microarray was used to detect changes in gene expression between CD 8 + T cells cultured with and without the SKOV 3 ovarian adenocarcinoma cell line. QRT‐PCR was performed to determine glycolysis gene expression in CD 8 + T cells from a transwell culturing system and OC patients. We also detected protein levels of glycolysis‐related genes using Western blot analysis. Results Comparing gene expression profiles revealed significant differences in expression levels of 1420 genes, of which 246 were up‐regulated and 1174 were down‐regulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis indicated that biological processes altered in CD 8 + Treg are particularly associated with energy metabolism. CD 8 + Treg cells induced by co‐culture with SKOV 3 had lower glycolysis gene expression compared to CD 8 + T cells cultured alone. Glycolysis gene expression was also decreased in the CD 8 + T cells of OC patients. Conclusions These findings provide a comprehensive bioinformatics analysis of DEG s in CD 8 + T cells cultured with and without SKOV 3 and suggests that metabolic processes may be a possible mechanism for CD 8 + Treg induction.