Open AccessmiR‐297 acts as an oncogene by targeting GPC 5 in lung adenocarcinoma
Author(s) -
Sun Yunchuan,
Zhao Jianyong,
Yin Xiaoming,
Yuan Xiangkun,
Guo Jianfei,
Bi Jianqiang
Publication year - 2016
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12288
Subject(s) - adenocarcinoma , microrna , cancer research , oncogene , carcinogenesis , cell growth , ectopic expression , lung cancer , biology , cell culture , cell , downregulation and upregulation , lung , microbiology and biotechnology , cancer , pathology , cell cycle , gene , medicine , genetics
Objectives Emerging studies have demonstrated that micro RNA s (mi RNA s) play crucial roles in carcinogenesis of many developing human tumours. However, the functions and mechanisms of miR‐297 in lung cancer have, up to now, been largely undefined. Materials and methods Here, miR‐297 expression was measured in lung adenocarcinoma tissues and cell lines, using qRT ‐ PCR . Lung adenocarcinoma cell line was treated with an miR‐297 mimic. MTT and colony analysis were performed to detect cell proliferation and colony formation. The direct target gene of miR‐297 was assessed by qRT ‐ PCR , Western blotting and luciferase assays. Results We demonstrated that miR‐297 expression was upregulated in lung adenocarcinomas compared to adjacent normal tissues. Expression of miR‐297 was also upregulated in tested lung adenocarcinoma cell lines. Ectopic expression of miR‐297 enhanced lung adenocarcinoma cell proliferation and colony formation. Furthermore, overexpression of miR‐297 promoted cell migration and invasion. In addition, we identified Glypican‐5 ( GPC 5) as a direct target gene of miR‐297 in lung adenocarcinoma cells. Expression of GPC 5 was downregulated in both lung adenocarcinoma tissues and cell lines. Moreover, expression of GPC 5 was inversely associated with expression of miR‐297 in lung adenocarcinoma tissues. Conclusions These results suggest that miR‐297 acted as an oncogenic mi RNA , partly by targeting GPC 5, adenocarcinoma of the lung.