Open AccessmiR‐375 controls porcine pancreatic stem cell fate by targeting 3‐phosphoinositide–dependent protein kinase‐1 (Pdk1)
Author(s) -
Hu Shuxian,
Zhang Mingzhi,
Sun Fen,
Ren Lipeng,
He Xin,
Hua Jinlian,
Peng Sha
Publication year - 2016
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12263
Subject(s) - microbiology and biotechnology , microrna , cell growth , transfection , stem cell , phosphoinositide 3 kinase , biology , cell cycle , islet , cellular differentiation , protein kinase b , kinase , signal transduction , cell , chemistry , gene , biochemistry , insulin , endocrinology
Objectives miR‐375 is one of the highly expressed micro RNA s (mi RNA s) found in pancreatic islets of both humans and mice. In this study, we investigated functions of mi RNA miR‐375 in porcine pancreatic stem cells ( PSC ). Materials and methods We transfected mimic and inhibitor of miR‐375 in PSC s to measure functional roles of the micro RNA and its effects on cell cycle proliferation and cell differentiation were determined. Luciferase assays were also performed to reveal the target gene of miR‐375. Results Overexpression of miR‐375 suppressed proliferation, promoted apoptosis and inhibited differentiation into islet‐like cells. PDK 1 was identified as being a target of miR‐375. Furthermore, we found that overexpression of miR‐375 inhibited activation of the PDK 1‐ AKT signalling pathway. Conclusion miR‐375 directly targeted PDK 1 in porcine PSC s, suppressing cell proliferation and differentiation into islet‐like cells.