Open AccessEstablishment and adipocyte differentiation of polycystic ovary syndrome‐derived induced pluripotent stem cells
Author(s) -
Yang Sheng,
Ding Shufang,
Jiang Xianglong,
Sun Bolan,
Xu Qianhua
Publication year - 2016
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12258
Subject(s) - induced pluripotent stem cell , polycystic ovary , biology , adipogenesis , cellular differentiation , embryonic stem cell , microbiology and biotechnology , adipocyte , stem cell , directed differentiation , endocrinology , adipose tissue , insulin , genetics , gene , insulin resistance , mesenchymal stem cell
Objective To establish a new biological cell model and approach to mimic abnormal lipid metabolism of polycystic ovary syndrome ( PCOS ) in vitro . Materials and methods Epithelial cells from PCOS patients were reprogrammed to pluripotency by retroviral transduction using defined factors. Morphology, growth characteristics, karyotype, gene expression and differentiation in vitro and in vivo were detected by identification protocol of human embryonic stem cells ( ESC s). PCOS ‐induced pluripotent stem cells ( iPSC s) were then induced to differentiate into adipocytes. Ability of the adipocytes for glucose consumption was compared with those from non‐ PCOS ‐ iPSC s. Results iPSC s were successfully generated from PCOS patients' adult cells. Formed iPSC clones had the same characteristics of human ESC s. PCOS ‐ iPSC s were induced to differentiation into normal karyotype adipocytes. Compared to non‐ PCOS ‐ iPSC s, PCOS ‐ iPSC s had more glucose consumption ability during adipocyte differentiation and development in vitro . Conclusions This protocol provides a new biological cell model and approach for studying pathogenesis of PCOS and discovering potential drugs to treat it.