Open AccessComparison of P 75 NTR ‐positive and ‐negative etcomesenchymal stem cell odontogenic differentiation through epithelial–mesenchymal interaction
Author(s) -
Xing Yongjun,
Nie Xin,
Chen Guoqing,
Wen Xiujie,
Li Gang,
Zhou Xia,
Tian Weidong,
Liu Luchuan
Publication year - 2016
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12248
Subject(s) - dmp1 , in vivo , biology , stem cell , microbiology and biotechnology , immunology , virus , viral matrix protein
Objectives The aim of this study was to investigate differences of odonto‐differentiation between P 75 ‐neurotrophin receptor (P 75 ‐ NTR )‐positive ectomesenchymal stem cells ( P 75 +EMSC s) and P 75 ‐ NTR ‐negative ectomesenchymal stem cells ( P 75 −EMSC s), and their underlying mechanisms. Materials and methods Primary cranial neural crest‐derived cells ( CNC ) were isolated from the first branchial arches, and P 75 +EMSC s and P 75 −EMSC s were sorted by fluorescence‐activated cell sorting. Differentiation of P 75 +EMSC s or P 75 −EMSC s into odontoblast‐like cells was induced by dental epithelial cells in vitro or in vivo . Differential gene expression profiles between P 75 +EMSC s and P 75 −EMSC s were analysed by microarray assay. Smad4‐specific small interfering RNA and activator kartogenin were used to treat the cells, to evaluate effects of Smad4 in odonto‐differentiation of P 75 +EMSC s or P 75 −EMSC s. Results Under induction of dental epithelium conditioned medium, P 75 +EMSC s had more mineralized node formation and higher expression of Dmp1 and Dspp compared to P 75 −EMSC s. In our in vivo study, graft of P 75 +EMSC s recombination with dental epithelium showed higher expression of DMP 1 and DSP . Knock‐down of Smad4 in P 75 +EMSC s significantly downregulated expression of DMP 1 and DSP , while activation of Smad4 in P 75 −EMSC s by the activator kartogenin, significantly increased DSP and DMP 1 expression. ConclusionsP 75 +EMSC s showed more odonto‐differentiation potential than P 75 −EMSC s both in vivo and in vitro . Smad4 played a critical role in determination of odonto‐differentiation potential of CNC ‐derived EMSC s.