Open AccessmiR‐142‐3p inhibits cancer cell proliferation by targeting CDC 25C
Author(s) -
Cao XuChen,
Yu Yue,
Hou LiKun,
Sun XiaoHu,
Ge Jie,
Zhang Bin,
Wang Xin
Publication year - 2016
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12235
Subject(s) - cell growth , cell cycle , ectopic expression , cancer , cell culture , biology , cell , cancer research , microrna , cancer cell , transfection , suppressor , cell cycle checkpoint , microbiology and biotechnology , gene , genetics
Abstract Objectives Micro RNA s (mi RNA s) contribute to control of cell cycle progression and are frequently deregulated in cancer. The focus of this study was to determine effects of miR‐142‐3p on the cell cycle progression and cancer cell proliferation. Materials and methods RT ‐ qPCR was performed to determine expression of miR‐142‐3p in a range of cancer cell lines and in clinical cancer specimens. To further understand its role, we restored its expression in cancer cell lines by transfection with miR‐142‐3p mimics or inhibitors. Effects of miR‐142‐3p on cell cycle progression and cell proliferation were also determined. Results miR‐142‐3p was down‐regulated in both cancer cell lines and cancer specimens. Its overexpression suppressed proliferation, whereas its depletion promoted it. In addition, miR‐142‐3p lead to cell cycle arrest in G2/M. Moreover, CDC 25C was identified as being a target of miR‐142‐3p, ectopic expression of which reversed suppression of cell proliferation. Conclusions Our observations suggest that miR‐142‐3p functioned as a tumor suppressor by targeting CDC 25C.