Open AccessCD 49f promotes proliferation of male dairy goat germline stem cells
Author(s) -
Zheng Liming,
Zhu Haijing,
Mu Hailong,
Wu Jiang,
Song Wencong,
Zhai Yuanxin,
Peng Sha,
Li Guangpeng,
Hua Jinlian
Publication year - 2016
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/cpr.12232
Subject(s) - stem cell , biology , downregulation and upregulation , embryonic stem cell , alkaline phosphatase , microbiology and biotechnology , andrology , biochemistry , gene , medicine , enzyme
Objectives CD 49f enhances multipotency and maintains stemness in embryonic stem cells ( ESC s), however, whether it would be effective in mGSC s has remained unclear. Moreover, better standards for mGSC enrichment and purification are necessary. The present study was conducted to determine roles of CD 49f in mGSC enrichment and regulation. Materials and methods CD 49f expression patterns were investigated in dairy goats. CD 49f positive cells were purified and enriched using magnetic‐activated cell sorting ( MACS ), and characteristics of the cultured cells were assayed using alkaline phosphatase ( AP ) analysis, quantitative real‐time PCR ( QRT ‐ PCR ) and immunofluorescence analysis. Furthermore, the exogenous CD 49f gene was transfected into mGSC s and its effects were analysed. Results CD 49f was found to be conserved in both mRNA and amino acid sequences and that it was an efficient marker for dairy goat mGSC identification, enrichment and purification. CD 49f positive cells expressed higher levels of mGSC ‐specific markers, and proliferated faster than CD 49f negative cells. Overexpression CD 49f promoted proliferation of dairy goat mGSC s, and Oct4 expression was upregulated; histone H3‐lysine 9 dimethylation (H3K9me2) was reduced. Conclusions Taken together, our data suggest that CD 49f plays novel and dynamic roles in regulating maintenance of pluripotency in mGSC s via Oct4 crosstalk and histone methylation dynamics,which may provide new solutions for mGSC s stability in vitro .